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文献和实验Embryo Dissection and Micromanipulation Tools
. All tools must be kept clean and rinsed with 70% ethanol to keep them sterile. RELATED INFORMATION For excellent advice on dissection and micromanipulation tools, see Hamburger (1960) . Forceps Stainless-steel forceps
a second coverslip on top, which will flatten the agarose into a thin pad. (try to avoid air bubbles, but a few won't hurt anything) When the agarose has hardened (> 5 sec) slide off the top coverslip. Use this top coverslip as the bottom coverslip to make
on top, which will flatten the agarose into a thin pad. (try to avoid air bubbles, but a few won't hurt anything) When the agarose has hardened (> 5 sec) slide off the top coverslip. Use this top coverslip as the bottom coverslip to make the next pad; its thin coating
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