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货号：T_70312513627
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英文名称：STAIN NU-CLEAR II ACDALC 4L
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文献和实验Preparation and Staining of Frozen Tissue Sections
for sectioning. II. Sectioning of Frozen Tissues Before cutting sections, allow the temperature of the block to equilibrate to the temperature of the cryostat (-20°C). Place the tissue block on the cryostat specimen disk. Adjust
in perfusion fixation ** Michel's fluid for transporting cells or specimens ** Microwave ovens: Advice for new users ** Paraformaldehyde: why won't it dissolve? ** Saccomano's fixative ** Zinc-containing fixatives: What has been published? ** Alternatives
tube and remove 5-10μl lysate for protein assay. Discard pellet or, if desired, resuspend in 50-200μl of 1X sample buffer, sonicate and boil. 5) If desired/necessary, pre-clear the lysate by adding 30μl protein A-, protein-G, or protein A/G-sepharose
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