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999
- 供应商：
北京泽平
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- 保修期：
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- 规格：
EA
货号：T_7016300-16
中文名称：
英文名称：POTASSIUM IODIDE,10% W/V,500ML
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文献和实验High‐Throughput Cytotoxicity Screening by Propidium Iodide Staining
(ATCC #CCL‐119), >95% viability determined by eosin exclusion (Hoskins et al., ) on a representative sample: passaged twice weekly in T175 tissue culture flasks at a cell density of 0.5 to 2 × 106 viable cells/ml (see appendix 3B ; quality of assay
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
a change in specific activity. 3.2. Preparation of Wnt3A CM for Fractionation 1. To the filtered CM from Section 3.1 , add Triton X-100 to1% (v/v), Tris-HCl, pH 7.5, to 20 mM, and NaN 3 to 0.01%(w/v) (e.g., to 930 mL CM, add 50 mL of 20% (v/v) TritonX
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