赛默飞世尔Thermo Fisher EDTA, 0.010

Clone Genes From a Phage Library

: This is a stabilization and storage solution for phage. 2.9g NaCl(50 mM), 1g Mg SO4(10 mM), 25 ml Tris pH 7.5 (50 mM), 50 mg gelatin (100 g/l) in 500 ml-- autoclave. • NaOH for plaque lifts: 0.5M NaOH, 1.5M Na Cl, 1 mM EDTA. • Tris for plaque lifts: 0.5M Tris HCl pH

从噬菌体文库中克隆基因

for several weeks.Use 50 microliters per plate. Filters: I like simple uncharged nylon filters from Fisher (MSI N00HY08250, 82 mm, 50/box). These are quite cheap. Solutions: LB, SM solution, Church hybridization solution, Church wash solution, Chloroform

Clone Genes From a Phage Librar

EDTA, 7 percent SDS, and 0.5 M NaPO4 (From a 1.0 molar stock, pH 7.2: 134g Na2HPO4/7H2O plus 4.5 ml of 85 percent phosphoric acid to one liter; pH carefully, or else your hybe solution will be viscous, and that is wrong). • Church wash: 50