赛默飞世尔Thermo Fisher EC HPLC-Tre

基本实验技术

of the buffer in the reaction is 1X. Ex. To set up a restriction digestion in 25 μl, one would add 2.5 μl of a 10X buffer, the other reaction components, and water to a final volume of 25 μl. B. Preparation of Working Solutions

【信息】Northstar BioProducts（NSBP）

with keratan sulfate, heparin or heparan sulfate     M.W.: 120,000-145,000 (gel filtration)        100332-1A Chondroitinase ABC Protease Free, (Proteus vulgaris), EC 4.2.2.4  2 units   Chondroitin ABC lyase (Chondroitin ABC eliminase). Lyophilized powder  

小RNA的Northern BLOT

. Note: 300mA is the max on our power supply. 5’end-labeling of Probe:  1 μl 10x PNK buffer (fresh from NEB)  30 pmol probe DNA oligo  1.66 μl [γ -32P] ATP, 150 mCi/ml (= 250 μCi, 42 pmol) Vial