赛默飞世尔Thermo Fisher X50 Membran

原创：western protocol

. Sponge - filter paper - gel - membrane - filter paper - sponge 3.  Transfer for 1 hr at 1 amp at 4°C on a stir plate. Bigger proteins might take longer to transfer. For the Mini-Transblot, it's 100 V for 1 hr with the cold pack and prechilled buffer

[分享] Strip Antibodies and Re-use Protein blots membrane

20, or overnight with 3% BSA/Tween 20. Five： Stripping buffer: 0.5 L (sterile filter solution and keep at 4°C) 0.2 M Glycine, pH 2.5 0.05% Tween 20 Six： 50mM tris pH6.8, 100mM b-mercapto and 2% SDS. Leave on for 30 mins at 50C and then wash

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

1.5 ml    1.2 mM EDTA 0.5M stock 120 µl    1.1% Triton X-100 10% stock 5.5 ml    0.01% SDS 10% stock 50 µl