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999
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货号：T_70189-002-901
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英文名称：ANTI-NDUFB6 MAXPAB 100UL
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文献和实验), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
Wholemount staining of embryos
5'' washes with TBS. Use haematology mixer (these are really good mixers Fisher cat#14-060-1) Block protein binding sites by incubating embryos in TBS plus 2 mg/ml BSA plus o.1 (v/v) triton-X-100 plus 20 heat inactivated normal serum (donkey or goat
In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
. Seydoux). 15) Dilute boiled probe in HB to give 100 ul of solution. Try a 1:3 dilution for probes to messages of unknown abundance. Experience with gld-1 probes suggests that probe dilution is not critical -- a strong, clean signal was obtained
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