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999
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北京泽平
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EA
货号:T_70114-360-458
中文名称:
英文名称:HIGH POLY CHAIR MED BK T C RTE
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文献和实验PCR PRIMER DESIGN AND REACTION OPTIMISATION
or C, respectively , to sequence inside the poly(A) region of cDNA clones of mRNA from eukaryotic origin. I have used it to amplify discrete bands from a variety of poly(A)+ virus RNAs, with only a single specific degenerate primer upstream: the T
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
horse serum (Invitrogen; heat-inactivated for 30 min at 60°C) NEP complete medium Neurobasal medium (PSG) Paraformaldehyde (PFA; Sigma), 4% in 1X PBS Poly-D-lysine (Sigma) Reconstitute in H2 O for a stock solution of 1 mg
after transplantation in the host striatum. (C ) EGFP‐positive SENA graft (green) immunostained for the neuronal marker protein NeuN (red). Note the high percentage of NeuN‐positive neurons in the SENA graft. Scale bars: 100 µm (B), 50 µm (C
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