赛默飞世尔Thermo Fisher UREA USP 50

Basic procedures for bacteria culture

microcentrifuge tube, invert to mix, and incubate in an ice-water bath for at least 10 minutes. It is possible to place the sample at -20degC overnight at this stage. 2. Centrifuge at 12,000 rpm in a microcentrifuge (Fisher) for 15 minutes at 4 degC, decant

小RNA的Northern BLOT

-833) Temed (BioRad cat# 161-0800) SafetyCoat: Baker cat# 4017-01 10% APS, Make a 10% w/v in DEPC treated H2O. Ammonium persulfate, Sigma A7460. Keep in refrigerator, make fresh every week. 10xTBE: (890 mM) 216g Trisbase Fisher

SDS-PAGE

staining your gel. The stain is: 1 g Coomassie brilliant blue R-250 50 ml glacial acetic acid 500 ml MeOH 450 ml H20 (Note: This is a somewhat weak stain recipe: it destains faster and gives a prettier gel, but may be less sensitive