赛默飞世尔Thermo Fisher TLC SILICA

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

) 5 × 20–cm glass chromatography columns (Labglass) 2.5 × 7.5–cm TLC plates precoated with a 250‐µm layer of silica gel 60 F 254 (EMD

Labeled Sphingomyelin Synthesis

of reaction by running a few microliters of the reaction mix on TLC (solvent system = chloroform/methanol/ammonium hydroxide @ 60:35:8). 4) Once reaction is complete, dry the mixture as much as possible using nitrogen gas. --> Should be left

Labeled Sphingomyelin Synthesis

standard onto a TLC plate. 18) Run TLC in chloroform/methanol/ammonium hydroxide (60:35:8) and develop spots using iodine vapor then potassium permanganate spray. --> Fraction (d) & (e) should contain unreacted DMSM, and fraction (f) should contain