赛默飞世尔Thermo Fisher X1000 CRIMP

常用缓冲液配制－2

into several small screw-cap jars (~50 ml capacity) Store at room temperature  20 x Energy Regeneration System* 150 mM creatine phosphate (Boehringer #127574) 2 ml of 100 mM MgATP Stock (20 mM ATP, 20 mM MgSO4 ) 40ml 0.5 M EGTA (2 mM EGTA)

Prime‐, Stress‐, and Cue‐Induced Reinstatement of Extinguished Drug‐Reinforced Responding in Rats: Cocaine as the Prototypical Drug of Abuse

10‐ml bottle heparin sodium (1000 U/ml concentration) Glycerol USP (Fisher Scientific or similar)

Stripping Western Blots

(from R. Plattner & A.M. Pendergast) 1. Following stimulation, place cells on ice and wash 2X with cold PBS 2. Lyse each 100 mm plate with 500-1000 μl of lysis buffer (50mM HEPES pH7.0, 150 mM NaCl, 10% glycerol, 1% triton-X-100, 1.5 mM MgCl