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- Thermo Fisher
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- T_70119-032-1013J
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70119-032-1013J
中文名称：
英文名称：PROBE 36IN HOLLOW BRASS TUBE
货期：热销品现货，其他请咨询
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文献和实验In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
at dilutions between 1:2 and 1:5, but it began to fall off at 1:10. Probe to major sperm protein RNA could be diluted 1:20 without any fall off in signal. 15a) Boil diluted probe 5 min, cool to 48°, and add to gonads. 16) Hybridize for 12 to 36 hr at 48
Northern Blotting方法(Howell Lab)
Fixing Bake membrane in 80℃ vacuum oven for 20 min. UV crosslink (on optimal setting of Fisher Crosslinker) the membrane (face side up). Store between 2 pieces of chromatography paper in a plastic bag. Labelling the probe
RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes
tablet (#B5655) ; Dissolve one tablet in 10 ml staining solution. If there is a non-specific background signal, you can dilute more (up to 20 ml). Culture tubes : Available from Fisher as 6 mm (ID) x 50 mm (length
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