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- 库存：
999
- 供应商：
北京泽平
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热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70108-772-129
中文名称：
英文名称：384-WELL WHT ASSAY 120UL 50/CS
货期：热销品现货，其他请咨询
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
for 25-50 nM final concentration.3.Count cells, then spin to pellet (~1200 rpm, 5').4.Resuspend cells at 1-5 x 106 cells/ml in serum free media.5.Plate 1 ml cells into wells of 6-well plate.It doesn't seem to matter if dsRNA or cells are added
What is your Protocol for RNAi on Cell Cultures
may vary depending on assay. 384-Well Plates Bathing Remove 384-well plates pre-aliquoted with dsRNA from freezer to thaw. The 384-well plates contain 5ul of ~0.05ug/ul dsRNA in water
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