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文献和实验Procedures for In Vitro Production of Bovine Embryos
1. Prepare embryo culture medium (modified KSOM) at least 2 h before removing embryos/oocytes from the fertilization drops.2. Make enough 50 ml microdrops of culture medium (30 oocytes/embryos per drop) on 60 x 15 mm petri dishes and cover with mineral oil
【转贴】immunofluorescence staining
m l buffer for 24 well trays, 1 ml for 6 well trays, etc.) Remove formaldehyde fix, discard in toxic waste bottle, and wash X 3 with PEM buffer, about 2-5 min. per wash.Incubate 5-30 min. with PEM + 0.5% Triton X-100. This step permeablizes the cells
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
with ice in the sterile culture hood. 2) Place a 250 mL beaker with 30-40 mL of Betadine into the hood. 3) Add 50 mL/litter of PBS-glucose into a bottle, seal and place into a 37°C water bath. 4) For each person
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