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- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
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热销品大量现货，其余请咨询
- 保修期：
1年
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EA
货号：T_70114-386-347
中文名称：
英文名称：FINNPIPETTE F2 5000UL FIX VOL
货期：热销品现货，其他请咨询
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文献和实验AFLP: not only for fingerprinting, but for positional cloning
. Run gel, dry (DO NOT FIX), and generate an autorad as per normal (see Sec. 3.0). 3) Cut out band of interest from dried gel (using autorad as the guide) and isolate the DNA via the "crush & soak" method of Sambrook et al. (Vol 1, pp. 6.46-6.48
AFLP: not only for fingerprinting, but for positional cloning
products to 0.3 M Na-acetate and add 2.5 vol EtOH, incubate @ -20° C for _ 20 min, then spin @ 12K x g for 5 min. Resuspend each DNA in just enough 2x formamide buffer to load into 1 lane on a standard PA-AFLP gel. Run gel, dry (DO NOT FIX), and generate
1) Wash cells twice with PBS and lyse in appropriate lysis buffer; Dish size Wash vol. Lysis vol. 1 well 1ml 125-250μl 6cm plate 3mls 250-500μl 10cm plate 5mls
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