赛默飞世尔Thermo Fisher X6 SYRINGE,

Loss of Heterozygosity

µl of 10% ammonium persulfate to 75 ml of Gel-mix-6. Mix by inversion. Hold the nozzle of the bottle at the corner of the gel cast. Hold the gel cast at a 45o angle to the bench and pour the gel between the plates. If bubbles get trapped

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

of aggregation plate Materials:       Dissecting microscope;       Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3);       1 ml syringe with 26 G 1/2 needle of KSOM medium;       light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);       

Random Primer DNA Labeling

for use later when determining total radioactivity. 8.Place a small amount of glass wool at the bottom of a 1 mL syringe. 9.Load sephadex G-50 (saturated in TE pH 7.4, 0.2% SDS) into the 1 mL syringe. 10.Pack the mini-column by spinning the 1 mL