赛默飞世尔Thermo Fisher EXTRACTION

RNA Extraction from Frozen Tissue Sections

laboratory practice when handling Trizol, as it is toxic and corrosive (i.e., wear gloves, lab coat, eye protection). Also label a 14 ml round bottomed polypropylene centrifuge tube (Falcon #2059) for each sample.   2.      In a cryostat at -20°C, cut

ChIP-Chip E. coli

DNA (see above) Incubate for 90 min at 42°C add 20 μl Proteinase K to each tube and divide on PCR tubes fitting your PCR machine (for example 2x100μl). (Note: many protocols no longer have this step; I get equivalent data

张峰手稿：基因工程技术之CRISPR-Cas9

used for PCR in this protocol. 4. Cloning enzymes: FastDigest Bbs I, FastDigest Age I, FastAP(Thermo Scientifi c/Fermentas, Pittsburg, PA, USA), Plasmid-Safe exonuclease (Epicentre, Madison, WI, USA), T7 DNA ligase. 5. 10× T4 DNA ligase buffer