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- 2025年07月07日
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- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70121-176F
中文名称：
英文名称：MICRODISPENSER FIX-V 100/200UL
货期：热销品现货，其他请咨询
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文献和实验Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
) DMEM/F12 (Invitrogen, Catalog # 12500-096 or equivalent) Materials: BG01V human embryonic stem cells Tissue culture dishes (60 mm; Fisher, Catalog # 08-772B, 100 mm; Fisher, Catalog # 08-772E, or equivalent) 15 mL conical
Wholemount staining of embryos
5'' washes with TBS. Use haematology mixer (these are really good mixers Fisher cat#14-060-1) Block protein binding sites by incubating embryos in TBS plus 2 mg/ml BSA plus o.1 (v/v) triton-X-100 plus 20 heat inactivated normal serum (donkey or goat
In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
at room temperature as follows: 2 x 2 min PBTw 3 x 20 min PBTw / BSA 2 x 5 min in Staining Buffer (see below). 24) Transfer gonads to staining buffer containing 4.5 ul NBT/ml, 3.5 ul X-phosphate/ml and 100 ng/ml DAPI and protect from light
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