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英文名称:TUBE W/CAP ROUND BOTTM 1000/PK
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文献和实验Procedures for In Vitro Production of Bovine Embryos
in front of the heater) - Place 1-3 straws of semen in citothaw. - Layer semen on top of Percoll gradient. - Place Percoll tube in a warmed centrifuge canister. - Centrifuge for 15 min at 1000 x g. - Collect semen pellet with a Pasteur pipet
RNA purification using TRizol--TRIZOL法提取RNA
of chloroform to each tube.b.Cap each tube. Shake samples vigorously by hand for 15 seconds.c.Incubate samples for 5 minutes at room temperature.d.Centrifuge samples for 15 minutes at 12,000 x g at 4oC.3. RNA Precipitation a.Transfer the upper aqueous phase
Southern Hybridization Protocols
). Mix gently by pipetting up and down and cap the tube. Spin for a few seconds in a microcentrifuge to bring the contents to the bottom of the tube.Incubate at 37℃ for 10 minutesStop the reaction by the addition of 2 m l 0.5% EDTA. For use
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