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- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70315143027
中文名称：
英文名称：Heater block insert SHB50ML Stuart 3 x 50mL
货期：热销品现货，其他请咨询
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文献和实验Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
off the nitrocellulose membranes and submerse in pyrex dish with 1 x PBS + 0.1% Triton X-100 (1 liter = 50ml 20x PBS + 979 ml dH2 O + 1ml Triton X-100). Wash with gentle shaking for 3 x 5 minutes at room temperature to remove agarose. Block with PBS + 2% BSA
In Situ HYBRIDIZATION TO TISSUE SECTIONS
remedies: The blade may be dirty. Carefully (with upward strokes) clean it with xylene. Or the knife may be at a bad angle to the block. Adjust it until the problem is solved or until it is obvious the knife angle isn't the problem. Or the knife
can be done overnight at RT with constant agitation. 3) Washed stripped membrane at least 3x for 10min each with PBST, then re-block (in PBST+NFDM or PBST+BSA) overnight at 4oC. Stripping Buffer: 62.5mM Tris pH 6.8 2% SDS 100mM b-ME Quick Mix
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