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文献和实验, electroelution, or binding of the DNA to silica particles or ion-exchange resins give quite pure DNA , but yields are relatively low. The apparently poor yield of DNA recovered by the spin techniques has been found to be clean enough for ligating directly
of the DNA to silica particles or ion-exchange resins give quite pure DNA, but yields are relatively low. The apparently poor yield of DNA recovered by the spin techniques has been found to be clean enough for ligating directly from the eluate without organic
Recovering DNA from agarose gels
's column discusses the pros and cons of various methods used to extract DNA fragments directly from agarose gels. For details on how to partake in the newsgroup, see the accompanying box. DNA electrophoresed through agarose gels is frequently used
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