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999
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北京泽平
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货号：T_70108-771-12
中文名称：
英文名称：CELL CULTURE INS 8.0UM 48/CS
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文献和实验Aggregation of ES Cells and Eight Cell Stage Embryos
to obtain in numbers. The ES cells are intorduced into the developing embryo by the adherance of the cells to a dezonulated 8 cell stage embryo. The embryos are cultured to blastocysts and then transfered into a pseudopregnant hybrid (C57 X CBA) F1 recipient
converted from fibroblasts. Nature communications, 2016, 7.1: 10080. 【6】Choi H, Shinohara M, Ibuki M, et al. Differentiation of human-induced pluripotent stem cell-derived endocrine progenitors to islet-like cells using a dialysis suspension culture
per well into 12 well plates with 1.5 mL/well of Iscove's transduction media. 3. Incubate 48 hrs at 37°C, 5% CO2 . 4. Harvest cells by spinning at 1000 rpm for 5 min. 5. Add 0.5 mL of 3x growth factors to each well (depending on the experiment).
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