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- 文献和实验
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999
- 供应商：
北京泽平
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热销品大量现货，其余请咨询
- 保修期：
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货号：T_70311660039
中文名称：
英文名称：X50 CULTURE TUBE GLASS 20X150+CAP
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
Eccles:Protein Lysates from Cells in Culture
. Dilute 20 fold in cell lysis buffer Cell Lysis Buffer containing 1x Complete Protease Inhibitor 50uL 20x Complete protease inhibitor 950uL Cell Lysis Buffer Harvest cells into 15mL tube by typsinisation as usual
, label culture dishes. For 1 type of tissue received, set up 8 dishes. For 2 types of tissue received, set 6 on each; for 3 or more types of tissue, set 4 on each. Centrifuge the tissue in collagenase for 6 minutes at 150 X g (900 rpm in Sorval GLC-2B
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