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文献和实验Cool growth competent E . coli
10 minutes. -Transfer to centrifuge bottle; spin 2500 x g , 10 min, 4°C. -Resuspend pellet in 80 ml ice-cold TB; place on ice for 10 min. -Spin again 2500 x g , 10 min, 4°C. -Resuspend in 20 ml ice-cold TB. -Add DMSO
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
, and the reaction is carried out at 70°C. Subsequently, workup, isolation, and purification lead to the requisite O 6 ?alkyl or O 6 ?aryl ethers in good to excellent yields. Curr. Protoc. Nucleic Acid Chem. 49:1.26.1?1.26.16. © 2012 by John Wiley & Sons
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