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货号：T_70312477960
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英文名称：CHROMATOGRAPHIC TUBE SPARE C 16/100 GLASS
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文献和实验Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
) Digested DNA is extracted once with phenol/chloroform, once with chloroform, precipitated with 3 volumes of 100% EtOH, and resuspended in TE at a final concentration of 100-200 µg/ml. c) The following reagents are mixed in 0.5 ml eppendorf tube. water
In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
Solution: 100 mM NaCl; 5 mM MgCl2 ; 100 mM Tris (pH 9.5); 0.1% Tween 20; 1mM Levamisole. Levamisole is a potential inhibitor of endogenous phosphatases. Culture tubes: Available from Fisher as 6 mm (ID) x 50 mm (length) in either Pyrex or borosilcate
RNA purification using TRizol--TRIZOL法提取RNA
of chloroform to each tube.b.Cap each tube. Shake samples vigorously by hand for 15 seconds.c.Incubate samples for 5 minutes at room temperature.d.Centrifuge samples for 15 minutes at 12,000 x g at 4oC.3. RNA Precipitation a.Transfer the upper aqueous phase
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