赛默飞世尔Thermo Fisher ANTI-JO-1 E

（共享）ELISA原理与实验方法

is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk

（交流）细胞ELISA

Cellular ELISA ProtocolFormalin Fixed Cell Plates1. Trypsinize confluent flasks2. Pool and count cells3. Centrifuge at 1500 rpm for 10 minutes4. Resuspend to the appropriate concentration in complete medium4 x 105 cells/ml for epithelial cells2 x

Cellular ELISA Protocol

2 x 105 cells/ml for fibroblast cells 5.     Add 100 ml/cell to 96 well culture plates. 6.     Incubate overnight at 37oC. 7.     Wash plates twice with PBS 8.     Add 125 ml/well 10% Buffered Formalin 9.     Fix for 15 minutes at room temperature