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货号：T_70114-380-802
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文献和实验ES CELL DNA EXTRACTION: TUBE ME
phase). 6. Mix the contents of the tube until an emulsion forms. (Shake by hand for ~1 min, (or vortex ~2sec),because vortexing shears long (genomic) DNA ). 7. Centrifuge 5-10 min in a microfuge at TRm. (The phases
ES CELL DNA EXTRACTION: TUBE ME
into the organic phase). 6. Mix the contents of the tube until an emulsion forms. (Shake by hand for ~1 min, (or vortex ~2sec),because vortexing shears long (genomic) DNA ). 7. Centrifuge 5-10 min in a microfuge at TRm. (The phases should be well-separated
ES CELL DNA EXTRACTION: TUBE METHOD
of each well into a separate 1.5ml eppendorf tube (µ-cent. tube). 4. Continue lysing the cells for 2 more hours. (60°ree;C). (Float tubes in waterbath- don't need tupperware). 5. Add an equal volume (250 µl) of phenol:chloroform (phenol: chloroform: isoamylalcohol, 25
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