赛默飞世尔Thermo Fisher MOLY FL .25

secondary antibody review -- data from 99 publications

ref host class subclass conjugation experiment dilution detail supplier 1 goat     Alexa Fluor 488 immunocytochemistry 1:250 localization of tagged EVA-1 in 293T cell Molecular Probes 1 goat     Alexa Fluor 546 immunocytochemistry 1:250

Southern Hybridization Protocols

8.0 20X SSC 50X Denhardts 20% SDS *SS DNA 10 mg/ml16.00 ml 1.25 ml 0.50 ml 6.25 ml 0.50 ml 0.25 ml 0.25 ml 25.00 ml- 50 mM Tris 10mM EDTA 5 x SSC 1 x Denhardts 0.2 SDS 100 m g/ml* Boiled for 10 min then cooled on ice. Then added to the solution at 65

常用缓冲液配制－2

into several small screw-cap jars (~50 ml capacity) Store at room temperature  20 x Energy Regeneration System* 150 mM creatine phosphate (Boehringer #127574) 2 ml of 100 mM MgATP Stock (20 mM ATP, 20 mM MgSO4 ) 40ml 0.5 M EGTA (2 mM EGTA)