赛默飞世尔Thermo Fisher X1000 VIAL
INSERT,200µL, glass, tapered, for narrow neck vials5 x 30mm (PACK OF 1000) 货号：T_70310728544- ￥1200 - 24000
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- 2025年07月10日
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- 库存：
999
- 供应商：
北京泽平
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热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310728544
中文名称：
英文名称：X1000 VIAL INSERT,200µL, glass, tapered, for narrow neck vials5 x 30mm (PACK OF 1000)
货期：热销品现货，其他请咨询
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
Northern Blotting方法(Howell Lab)
for at least 30 min before quantitation. Pipet up and down several times before taking 2 µl into 998 µl water. Read at 260 nm. RNA concentration (µg/µl) = OD x 500 x 0.04 µg/µl = OD x 20 Formaldehyde gel Boil 2 g agarose in 144 ml
to the column and wash the reaction vial with an additional 200-300 µl of fresh TEAB. Add the wash to the column and allow it to begin to run down into the matrix. When the dye product has been absorbed into the matrix, refill the column with buffer and reattach
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