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- 库存:
999
- 供应商:
北京泽平
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- 保修期:
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- 规格:
EA
货号:T_701FA10019G
中文名称:
英文名称:PIPETMAN L FIXED, FL5
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文献和实验Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
capillary cutter or a 26‐G needle attached to a 3‐ml syringe or a sterilized ultra sharp splitting blade Micropipet tips 20‐µl
: Use 4X stacking buffer at 1:8, 10% SDS at 1:5, and b-ME at 70μl/10ml (e.g.10mls strip=1.25mls 4X, 2mls 10% SDS, 70μl b-ME & 6.68mls H2O) Lysis of Cultures Cells General Protocol 1) Wash cells twice with ice-cold PBS. Use 3mls (6cm plate
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