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- 技术资料
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999
- 供应商：
北京泽平
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EA
货号：T_70105-719-362
中文名称：细菌瓶黄色
英文名称：BOTTLE STERILE BACT 250/CS
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文献和实验Cool growth competent E . coli
al., 1990). Chill the following: >100 ml ice-cold TB, centrifuge (GSA rotor), 500 ml centrifuge bottle, and sterile freezing tubes (eppendorf tubes w/ good caps) -Innoculate 250 ml SOB media (2 liter flask) w/ 10-12 colonies bacteria
Preparation of Embryonic Extract
bottle. 4. 2 sets of 100 mm sterile petri dishes (glass or plastic) on ice. 5. 1x 50 ml sterile beaker, keep on ice. 6. 3x 250 ml sterile beakers, keep on ice. 7. Cheese cloth, sterilized by autoclaving. 8. 250 ml Saline G, on ice
Procedures for In Vitro Production of Bovine Embryos
Needed 2 x 1 L sterile saline (transport saline) 1 aliquot of stock 16 (Pen-Strep 10 ml) per bottle of saline 3 to 4 bottles of oocyte collection medium (OCM) (400 ml) 1 aliquot of stock 11 (glutamine 4 ml) per bottle of OCM
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