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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70123-451-009
中文名称:
英文名称:ANTI-DSDNA ELISA 96/PK
货期:热销品现货,其他请咨询




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文献和实验is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
Cellular ELISA ProtocolFormalin Fixed Cell Plates1. Trypsinize confluent flasks2. Pool and count cells3. Centrifuge at 1500 rpm for 10 minutes4. Resuspend to the appropriate concentration in complete medium4 x 105 cells/ml for epithelial cells2 x
2 x 105 cells/ml for fibroblast cells 5. Add 100 ml/cell to 96 well culture plates. 6. Incubate overnight at 37oC. 7. Wash plates twice with PBS 8. Add 125 ml/well 10% Buffered Formalin 9. Fix for 15 minutes at room temperature
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