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- 库存:
999
- 供应商:
北京泽平
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- 保修期:
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货号:T_701138255
中文名称:
英文名称:ACETIC ACID, 25% V/V, 20 L
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文献和实验). 3. Tris-buffered saline (TBS) with Tween (TBS-T): Prepare10× stock with 1.37 M NaCl, 27 mM KCl, 250 mM Tris-HCl, pH 7.4, and 1% (v/v) Tween-20. 4. Blocking buffer: 4% (w/v) bovine serum albumin (BSA) and0.1% (w/v) sodium azide in TBS. 5. Primary
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
V294.PART II,Chapter 2 Boyden法体外趋化试验
of ethylene diamine tetraacetic acid (0.53 mM) and 0.01 g of phenolred in 1 L of phosphate-buffered saline, pH 7.4. 7. 2.5% (w/v) Trypsin (cat. no.15090-046; Gibco Invitrogen): dilute 1:25 in Versenebefore use. 8. Collagen from calf skin (cat. no. C
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