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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
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- 保修期:
1年
- 规格:
EA
货号:T_70313490557
中文名称:
英文名称:PT 1000.90 TEMPERATURE SENSOR, COATED
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文献和实验Immunostaining of Paraffin Sections
, in 400 ml of 50 µg/ml polylysine (Sigma #P1524; make up in 10 mM Tris, pH 8) for 30 min. Cover with foil and let dry overnight at room temperature. Polylysine solution can be stored at -20°C and reused. Store coated slides at 4°C and use
Immunohistochemisty-Enzymatic Protocol
by vascular perfusion with 500 - 700 mL of Fixative. Dissect the tissue. Immerse the tissue in 70 ethanol three times for 30 minutes each at room temperature. Immerse the tissue in 90 ethanol two times for 30 minutes each at room
1. Grow D3 mES cells in a gelatin-coated T25 tissue culture flask in a humidified, 5% CO2 incubator at 37ºC until 90%-95% confluent (2 d). D3 mES cells may also be grown in 10-cm, gelatin-coated tissue culture dishes or flasks. Other ES cell lines
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