赛默飞世尔Thermo Fisher FUNNEL AD W

Procedures for In Vitro Production of Bovine Embryos

(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15

AAV最新进展

从500ml的细菌培养物中纯化出来，其分为4´125m，每个部分用Qiagen Plasmid Maxi试剂盒的方法处理。 人293(Ad-5转化胎肾上皮)细胞和Hela细胞在补充有10%胎牛血清和抗体的EMEM中培养。细胞在37°C、5％CO2的单层培养基中培养。对照的包装实验感染倍数(m.o.i)为15的Ad5型在无血清培养基中培养1h，随即进行转染。Ad通过在Hela细胞上的噬斑形成滴定。 Fig.1. rAAV包装所用的质粒(A)pTR-UF5含有CMV启动子(箭头)所驱动的人gfp基因

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

. Chem. 67:3057‐3064.    van der Wenden, E.M., von Frijtag Drabbe Künzel, J.K., Mathôt, R.A.A., Danhof, M., IJzerman, A.P., and Soudijn, W. 1995. Ribose‐modified adenosine analogs