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- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311797567
中文名称:
英文名称:X1000 SCREW CAP 9MM PRECUT
货期:热销品现货,其他请咨询




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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
Northern Blotting方法(Howell Lab)
buffer. Pour GITC/Cell mix into (14x95 mm) polyallomer tubes with 4 ml CsCl buffer. Balance and top off tubes with more GITC buffer. Spin tubes on the SW40Ti rotor for 20 hr at 32,000 rpm (18℃). Pour off liquid and keep tubes
In vitro transcription reaction
-HCl, pH 7.5; 0.1 M NaCl; 10 mM EDTA). 2- Load transcription reaction on the column. Place screw cap tube to collect the radioactive probe. Pipette on top of the column 70 ml more STE and collect into the same tube. Mix and measure volume (~110-130 ml
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