赛默飞世尔Thermo Fisher MICROPIPETT
E ACURA 20-200µL micropipette 20 - 200µL 货号：T_70310292102- ￥1200 - 24000
- Thermo Fisher
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- T_70310292102
- 2025年07月06日
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- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310292102
中文名称：
英文名称：MICROPIPETTE ACURA 20-200µL micropipette 20 - 200µL
货期：热销品现货，其他请咨询
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文献和实验Method for Single-Cell Electroporation
. Efficiency of SCE was not noticeably effected by the ionic composition of the resuspension solution (2mM CaCl2, 20-200mM NaCl, or only dH2O) or DNA concentrations ranging from 0.1 to 5 µg/µl. DNA solution was introduced into the micropipette using either a 1
High efficiency LiAc transformation
and transfer the suspension to a 1.5 ml microfuge tube. 7. Pellet the cells at top speed for 15 sec and remove the LiAc with a micropipette. 8. Resuspend the cells to a final volume of 500 µl (2 x 109 cells/ml) -- about 400 µl of 100 mM LiAc-- Note
method is to physically break the needle. Over a Bunsen burner draw out a standard (not microinjection) 10 µl micropipette to about 1/5 its starting thickness. Place a stretch of the drawn out part on a 24x50 mm coverslip, and put a drop
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