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文献和实验Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
. For mammalian tubulins, the temperature is raised to 35-37¡C during observation. We have done this using warm air blowers or by wrapping the high NA condenser and objectives with thin silicone tubing through which hot water is circulated -- the temperature
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
with thin silicone tubing through which hot water is circulated -- the temperature of the water is empirically adjusted such that the temperature of the immersion oil on the coverslip surface is 35-37¡C (measured using a thermocouple probe). A variety of home-rigged
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