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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_7018125-1
中文名称:
英文名称:SULFOSALICYLIC ACID, 10%, 4 L
货期:热销品现货,其他请咨询




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文献和实验Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
150 µl 20% SDS400 µl 10% AP20 µl TEMEDMix gently and pour between plates up to ~1.5 inches below the top, depending on your apparatus. (A rule of thumb: want the stacking gel to be about as deep below the wells as the wells are deep). Immediately
. 2 ml 1 M KCl 1 ml 1 M Tris-HCl, pH 7.6 1 ml 1 M MgCl2 0.1 ml 1 M DTT ddH2 O to 10 ml RNase T1 : 100 U/ul in 50 mM Tris-HCl, pH 7.6 100 μl RNase T1 (Sigma R-8251) (100,000 U/0.2 ml) 25 μl 1 M Tris-HCl, pH 7.6 375 μl ddH2 O 500 μl 10%
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