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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：61849
中文名称：
英文名称：200ul Tip ART barrier MPRacked 10 trays/pack
货期：热销品现货，其他请咨询
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文献和实验ES / MEF cell culture and electroporation of targeting construct
(40ug of clean linear DNA in 1 X TE @ 1 ug/ul for each electroporation). Mix the cells and DNA with a 200-1000 ul barrier tip trying not to create bubbles. Add 400 ul of the cell/DNA mixture to a BTX cuvette. Set Electroporator as follows: 500
ES / MEF cell culture and electroporation of targeting construct
of cells to a microfuge tube containing the targeting construct (40ug of clean linear DNA in 1 X TE @ 1 ug/ul for each electroporation). Mix the cells and DNA with a 200-1000 ul barrier tip trying not to create bubbles. Add 400 ul of the cell/DNA mixture
ES Cell Culture and Manipulation
-well plate, be sure to make the 24-well feeders ahead of time. Trypsinize by rinsing with 0.5ml trypsin-EDTA, aspirate and add another 200µl trypsin for ~10 minutes. Add 800µl ES media with a lml pipette with a yellow tip and pipette several times to break
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