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- T_70122-045-317
- 2025年07月03日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70122-045-317
中文名称：
英文名称：FLUID CHAMBER ONLY 200/CS
货期：热销品现货，其他请咨询
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文献和实验AMNIOTIC FLUID CULTURES ON COVERSLIPS
Glacial Acetic Acid: FISHER cat # A38-500. Acetic acid, Glacial ACS (Aldehyde free), 500 ml bottle. L-Glutamine: GIBCO cat# 320-5030. L-Glutamine solution 100X (200 mM), 20 ml bottle, store frozen. Methanol
V294.PART II，Chapter 2 Boyden法体外趋化试验
vertically so that the end of the pipettip rests against the side of the well just above the membrane. Eject the fluid out of the pipet tip with a rapid motion to avoid trapping bubbles in the bottom ofthe well. 6. Wrap the whole chamber in plastic
6μg DNA (total) 20μl SuperFECT Vortex briefly & gently (~50% power) and incubate at room temperature for 20min. 3) Add complete media (DMEM+10% CS (NIH3T3, REF52) or MEM+10%FBS (WI38)) to each tube. 0.6ml / tube for 1 well 1.0ml / tube
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