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- 详细信息
- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
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- 保修期:
1年
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EA
货号:T_70311561315
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英文名称:300 ML GLASS FUNNEL 47MM DIAM
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
. A 50 ml culture typically yields enough RNA for 200 gel lanes (see below), and, as noted, smaller culture volumes may be used. 2. Flat mycelial pads are easier to grind than mycelial balls. Therefore, filter cultures using a Buchner funnel
A quick RNA mini-prep for Neurospora mycelial cultures
. 4. Working quickly before powder can thaw, pour or spoon ground mycelia into 15 ml round bottom Sarstedt tube (Sarstedt tubes #60.540, Princeton, NJ) containing 8 ml of E buffer at room temperature. [E buffer: 50 mM Tris-Cl pH 8.0, 300 mM NaCl, 5 mM
and lock box (from various vendors) Mini-centrifuge (VWR, 20668-212) Thermolyne Dri-Bath, Type 17600 (VWR, 35756-006) Platform Shaker, New Brunswick Scientific (VWR, 40000-300) Electrophoresis Separation Systems (Owl) QIAquick Nucleotide Removal Kit (Qiagen
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