赛默飞世尔Thermo Fisher SEROL. PIPE

Procedures for In Vitro Production of Bovine Embryos

with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap

SILVER STAINING OF NUCLEOLAR ORGANIZING REGIONS (NOR)

. Harleco Leishman Stain, 25 gram/bottle. Store in dark. Methanol: AMERICAN SCIENTIFIC PRODUCTS cat#3016-1. Methyl alcohol anhydrous AR (ACS)(Absolute) Acetone free, 500 ml bottle. Silver Nitrate: FISHER SCIENTIFIC CO. cat# S-181. Silver Nitrate

Tg 008 Southern Blotting

. 3. Roll membrane(s) between mesh into tube so that prehybridization buffer will coat all surfaces. 4. Add warmed prehybridization buffer to tube and incubate at 65℃ for at least two hrs. For large tube use 25-50ml prehybridization buffer depending