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- 文献和实验
- 技术资料
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999
- 供应商：
北京泽平
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- 保修期：
1年
- 规格：
EA
货号：T_70315163027
中文名称：
英文名称：Heating block clamp SHB250CLAMP Stuart medium
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文献和实验Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
of penicillin-streptomycin (same concentration as above) to 250ml sterile Dulbecco's Modified Eagle's Medium (DMEM) and bring the volume to 500 ml with sterile DMEM before sterile filtering through a 0.2 micron filter. 3) Sterilize surgical
Marcantonio Lab Protocol Manual
is determined by the desired number of time points. Reaction volumes can be scaled up or down in proportion to those described below. 1) Pre-warm a water bath to 30 degrees. Pre-heat a heating block to 70 degrees. Pour a 1% agarose minigel. 2) Remove
). Repeat 4-7, then clamp sides. 3. Add 0.12 ml of 10% ammonium persulfate solution and 12µl of TEMED (kept at 4°C) to the acrylamide solution prepared above. The APS solution should not be more than 1 week old. 4. Pour the acrylamide solution
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