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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311778398
中文名称:
英文名称:X50 HYBOND-N 82MM DISCS Hybond N, neutral nylon, sheets, discs 82mm (25006
货期:热销品现货,其他请咨询




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文献和实验bacteria Saran wrap Denaturing solution (0.5 M NaOH + 1 M NaCl) Neutralizing solution (1 M Tris pH 7.4, 1 M NaCl) 2X SSC, 0.1% SDS 83 mm circular nylon membranes (Hybond N) Stratalinker UV box Radiolabled probe
SOUTHERN BLOTTING OF GENOMIC DNA
to help turn over the gel.Use a wet,gloved finger to press out any air bubbies. • Place a sheet of Hybond N membrane on the gel.Use a piece the same size as the gel.Do not prewet the membrane.Be careful to align the membrane with the gel.Use a pipette
.Wear gloves for the following steps7. Cut 2 pieces of large 3MM paper (wicks) and 2 pieces about 2mm smaller than the gel on each edge and one piece of nylon (Hybond N , Amersham) the same size as the gel.Large gel (HFI) Medium gel Mini gel2 (14 x 19cm
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