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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70115-401-28A
中文名称:
英文名称:REAGENT CHROMIUM 250
货期:热销品现货,其他请咨询




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文献和实验Cloning by Limiting Dilution of Hybridoma
according to the calculated dilutions. Serially dilute each clone to 4, 2, and 1 cell/ml. The final dilution tube should contain 50 ml of cloning medium at 1 cell/ml. Pour each of the dilutions into a sterile reagent reservoir. Plate 250 ml/well into 96-well plates
Materials: 250 µL of fresh mouse blood in plastic tubes containing EDTA. RBC lysis buffer (388 mM NH4 Cl, 29.7 mM NaHCO3 , 25 µM Na2 EDTA) 20.75g. NH4 CL 2.5g NaHCO3 0.093g Na2 EDTA 1L H2
Immunohistochemisty-Enzymatic Protocol
Bacitracin (Sigma Catalog # B-0125), pH 7.2 PBS: 50 mM Na2HPO4 and 140 mM NaCl, pH 7.2 Incubation Buffer: 1 bovine serum albumin (Sigma Catalog # A2153), 1 normal donkey serum (Sigma Catalog # D9663), 0.3 Triton X-100 (Sigma Catalog # T9284
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