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货号:T_70315130519
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英文名称:Freezer rack for use with EXF600, HFU600B holds 14 tube boxes
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文献和实验Procedures for In Vitro Production of Bovine Embryos
ml Drummond Microdispensor (also purchased from Fisher ). For tips on how to use these instruments, click here .14. After oocytes have been cleaned of debris, transfer groups of 10 to a 50 ml microdrop of OMM (Fig. 13). Note: It is essential
buffer. 13. Centrifuge at 2880 x g for 5 minutes. 14. Discard flow-through and add another 10 ml of RPE buffer. 15. Centrifuge at 2880 x g for 10 minutes. 16. Put the column in a fresh 50 ml tube and add 1 ml of DEPC treated water
V294.PART II,Chapter 5 细胞粘附实验(英文版)
by centrifugation in a microfuge at 3800g for 15 min and then keepthe cell pellets in –20°C freezer. Simultaneous to step 7, add 600 μL of the substratesolution to the microtube, vortex, and then transfer the cell/substrate mixtureto new wells of the 96-well ELISA
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