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文献和实验Preabsorbing Antisera with C. elegans Acetone Powder
) Buchner funnel by placing a circle of Whatmann 3MM paper inside, and wetting the paper. 7. Pour the precipitate/acetone suspension into the funnel and filter it through. Wash through some more acetone. 8. Dry the material overnight at 37° spread
A quick RNA mini-prep for Neurospora mycelial cultures
. A 50 ml culture typically yields enough RNA for 200 gel lanes (see below), and, as noted, smaller culture volumes may be used. 2. Flat mycelial pads are easier to grind than mycelial balls. Therefore, filter cultures using a Buchner funnel
A quick RNA mini-prep for Neurospora mycelial cultures
. This should take about 5 minutes. 6. Add 1.1 ml of 3M KCl, invert to mix, keep on ice for 10 min. Solution should form semi-solid, flocculent mass as K-SDS precipitate forms. 7. Spin at 3000g, 4°C in a fixed angle rotor. Make sure caps are screwed on tightly to prevent
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