赛默飞世尔Thermo Fisher DUST-OFF RE

Aggregation of ES Cells and Eight Cell Stage Embryos

cylinder. The excess Sigmacote in the 10ml tube may be used to refill the syringe when empty. Once 50-80 pieces have been siliconized the glass is rinsed no less than eight times with Milli-Q water, shaking the water out of the glass by hand

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

Immunohistochemisty-Enzymatic Protocol

Bacitracin (Sigma Catalog # B-0125), pH 7.2 PBS: 50 mM Na2HPO4 and 140 mM NaCl, pH 7.2 Incubation Buffer: 1 bovine serum albumin (Sigma Catalog # A2153), 1 normal donkey serum (Sigma Catalog # D9663), 0.3 Triton X-100 (Sigma Catalog # T9284