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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310506282
中文名称:
英文名称:X12 Membrane Vivaspin 20 5-20ml samples MWCO (PES) 30,000 Polyethersulfone (pack of 12)
货期:热销品现货,其他请咨询




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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
), GSK-3b (dilution1:1,000; 1 h at RT), or b-actin (dilution 1:10,000; 1 h at RT). 5. Wash the membrane three times for 5 min each in TBS-T. 6. Incubate the membrane for 1 h at RT with 30 mL of TBS-Tcontaining anti-mouse or anti-rabbit IgG
Kodak X-OMAT AR film (XAR) (Fisher, 05-728-41) 14 x17 Kodak X-OMAT AR film (XAR) (Fisher, 05-728-42) 8 x 10 and 14 x 17 intensifying screen (Fisher, FB-IS-810 and FB-IS-1417) ART pipette tips (Rainin) Protocol DNA Preparation/Transfer to Membrane
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